EFI - Chemically-Guided Functional Profiling

This web resource is supported by a Research Resource from the National Institute of General Medical Sciences (R24GM141196-01).

The tools are available without charge or license to both academic and commercial users.

Reorganization of UniProtKB

With the current 2026_02 release, the UniProtKB database is reorganized to include an expanded number of Reference Proteomes to better capture biodiversity. This includes the removal of proteins from taxonomically unclassified organisms, i.e., those without a binomial species name (genus and species). The total number of accessions in UniProtKB has been reduced from 253,635,358 in the “legacy” 2025_03 release to 149,810,139 in the current 2026_02 release.

We are providing the option to select either the “legacy” 2025_03 database or the current UniProtKB database (now 2026_02) when generating SSNs. You can select the database in the “Database” accordion on the pages for the EFI-EST options, the EFI-GNT tool, and the Taxonomy Tool. We suggest that you compare the SSNs, GNNs, and GNDs generated from both databases as you explore the information you are seeking.

Because the “legacy” 2025_03 release contains UniProt IDs that are no longer active on the UniProt web site, we provide the Metadata Tool that provides access to the node attribute metadata for the UniProt IDs in the “legacy” 2025_03 release.

Markers Computation Results

Submitted SSN: 27073_26149_IP91_IPR004184_NoFragments_Firmicutes_UniRef90_NoFragments_IPR004184_Firmicutes_Minlen650_AS240_full_ssn_coloredssn

Submission Summary
Identified Markers

Submission Summary Table

Input filename	27073_26149_IP91_IPR004184_NoFragments_Firmicutes_UniRef90_NoFragments_IPR004184_Firmicutes_Minlen650_AS240_full_ssn_coloredssn.zip
Identify ID	837
Minimum sequence length	650
Identify search type	DIAMOND
Reference database	UNIREF90
CD-HIT identity for ShortBRED family definition	85

Markers that uniquely define clusters in the submitted SSN have been identified.

Files detailing the identities of the markers and which sequences they represent are available for download.

SSN With Marker Identification Results

The SSN submitted has been edited to include the marker ID and type and the number of markers that were identified.

	File	Size
	SSN with identify results (ZIP)

CGFP Family and Marker Data

The CD-HIT ShortBRED families by cluster file contains mappings of ShortBRED families to SSN cluster number as well as a color that is assigned to each unique ShortBRED family. The ShortBRED marker data file lists the markers that were identified.

	File	Size
	CD-HIT ShortBRED families by cluster
	ShortBRED marker data

Submission is currently disabled due to site maintenance. Submission is currently disabled due to site maintenance.

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