EFI - Enzyme Similarity Tool
This web resource is supported by a Research Resource from the National Institute of General Medical Sciences (R24GM141196-01).
The tools are available without charge or license to both academic and commercial users.
Please cite your use of the EFI tools:
Rémi Zallot, Nils Oberg, and John A. Gerlt,
The EFI Web Resource for Genomic Enzymology Tools: Leveraging Protein, Genome, and Metagenome Databases to Discover Novel Enzymes and Metabolic Pathways. Biochemistry 2019 58 (41), 4169-4182.
https://doi.org/10.1021/acs.biochem.9b00735Nils Oberg, Rémi Zallot, and John A. Gerlt,
EFI-EST, EFI-GNT, and EFI-CGFP: Enzyme Function Initiative (EFI) Web Resource for Genomic Enzymology Tools. J Mol Biol 2023.
https://doi.org/10.1016/j.jmb.2023.168018
Update of EFI Tools
The EFI Tools are being upgraded on 10/26/26. The site will not be available from
10/23-10/26 for maintenance.
While the updated site is functionally
identical to the old site, jobs run prior to that date will only be available
through the results link in the finalization email (not the Previous Jobs tab).
User accounts created on the old site will no longer be available, and users
are encouraged to create new accounts when the upgraded site becomes available.
A sequence similarity network (SSN) allows for visualization of relationships among
protein sequences. In SSNs, the most related proteins are grouped together in
clusters.
The Enzyme Similarity Tool (EFI-EST) makes it possible to easily generate SSNs.
Cytoscape is used to explore SSNs.
A listing of new features and other information pertaining to EST is available on the
release notes page.
InterProScan sequence search can be used to
find matches within the InterPro database for a given sequence.
Information on Pfam families and clans and InterPro family sizes is available on
the Family Information page.
EFI database version: 2026_02 / 109
Submission is currently disabled due to site maintenance.
Submission is currently disabled due to site maintenance.
Submission is currently disabled due to site maintenance.
Submission is currently disabled due to site maintenance.
Submission is currently disabled due to site maintenance.
Submission is currently disabled due to site maintenance.
Submission is currently disabled due to site maintenance.
UniProt Version: 2026_02
InterPro Version: 109
The family(ies) selected has proteins—this is greater than
the maximum allowed (75,000). To reduce computing time and the size of
output SSN, UniRef90 cluster ID sequences will automatically be used.
In UniRef90, sequences that share
≥90% sequence identity over 80% of the sequence
length are grouped together and represented by an accession ID known as the cluster ID. The output
SSN is equivalent a to 90% Representative Node
Network with each node corresponding to a UniRef cluster ID, and for which the node attribute
"UniRef90 Cluster IDs" lists
all the sequences represented by a node. UniRef90
SSNs are compatible with the Color SSN utility as well as the EFI-GNT tool.
Press Ok to continue with UniRef90.
This job will be permanently removed from your list of jobs.
Click here to contact us for help, reporting issues, or suggestions.
